Rapid induction of na/h exchange activity in barley root tonoplast.

نویسندگان

  • J Garbarino
  • F M Dupont
چکیده

Na(+)/H(+) exchange activity in barley (Hordeum vulgare cv CM-72) root tonoplast was induced by Na(+) even in the presence of inhibitors of protein synthesis. Induction occurred with a half-time of only 15 minutes. When salt-treated roots were transferred to a nutrient solution containing no Na(+), the activity disappeared with a similar time course. The data suggest that Na(+)/H(+) exchange was due to activation of an existing protein rather than to de novo protein synthesis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

NaCl Induces a Na/H Antiport in Tonoplast Vesicles from Barley Roots.

Evidence was found for a Na(+)/H(+) antiport in tonoplast vesicles isolated from barley (Hordeum vulgare L. cv California Mariout 72) roots. The activity of the antiport was observed only in membranes from roots that were grown in NaCl. Measurements of acridine orange fluorescence were used to estimate relative proton influx and efflux from the vesicles. Addition of MgATP to vesicles from a ton...

متن کامل

In vivo 23Na and 31P NMR measurement of a tonoplast Na+/H+ exchange process and its characteristics in two barley cultivars.

A Na+ uptake-associated vacuolar alkalinization was observed in roots of two barley cultivars (Arivat and the more salt-tolerant California Mariout) by using 23Na and 31P in vivo NMR spectroscopy. A NaCl uptake-associated broadening was also noted for both vacuolar Pi and intracellular Na NMR peaks, consistent with Na+ uptake into the same compartment as the vacuolar Pi. A close coupling of Na+...

متن کامل

Activity of tonoplast proton pumps and Na+/H+ exchange in potato cell cultures is modulated by salt.

The efficient exclusion of excess Na from the cytoplasm and vacuolar Na(+) accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by transmembrane transport proteins that exclude Na(+) from the cytosol in exchange for H(+), a secondary transport process which is energy-dependent and driven by the proton-motive force generated by plasma-me...

متن کامل

Na compartmentalisation in Salicornia

The tonoplast Na + /H + -antiporter and the tonoplast H + -pumps are essential components of salt tolerance in plants. The objective of this study was to investigate the transport activities of the tonoplast Na + /H + -antiporter and the tonoplast V-H + -ATPase and V-H + -PPase in a highly tolerant salt accumulating halophyte, Salicornia dolichostachya, and to compare these transport activities...

متن کامل

Tonoplast H+-ATPase activity in barley roots is regulated by ATP and pyrophosphate contents under NaCl stress.

The changes in ATP and PPi contents in roots under NaCl stress, and the effect of PPi on tonoplast H(+)-ATPase activity were studied with two barley cultivars differing in salt tolerance. The results showed that tonoplast H(+)-ATPase activity in salt-tolerant barley cultivar "Tanyin 2" roots increased obviously under NaCl 200 mmol/L (containing half-strength Hoagland solution) for 2 d, and decr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 89 1  شماره 

صفحات  -

تاریخ انتشار 1989